There were no children of Nathaniel's first marriage to the former Lucy Empshall, who died in December 1843.
At the age of about 44 years, on 9 December 1848, in York, Yorkshire, England, Nathaniel HORSEFIELD was sentenced to 15 years and transportation for burglary. He arrived in Fremantle on 25 October 1850 on the Hashemy and obtained his Ticket of Leave on the same date. On 17 February 1852, he obtained his Conditional Pardon.
At the Murchison House Station Cemetery, the gravestone which was erected in memory of Nathaniel Horsfield was erected by his master and fellow servants.
The Murchison House Station Cemetery record states that Nathaniel Horsfield was a botanist brought to the station by the original owner, Charles von Bibra, who died in October 1869.